2014年 | Haruo Sugi, Shigeru Chaen, Takakazu Kobayashi, Takahiro Abe, Kazushige Kimura, Yasutake Saeki, Yoshiki Ohnuki, Takuya Miyakawa, Masaru Tanokura, Seiryo SugiuraDefinite Differences between In Vitro Actin-Myosin Sliding and Muscle Contraction as Revealed Using Antibodies to Myosin Head. PLos One. 2014 9(6): e93272 |
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2013年 | Haruo Sugi, Takahiro Abe, Takakazu Kobayashi, Shigeru Chaen, Yoshiki Ohnuki, Yasutake Saeki, Seiryo SugiuraEnhancement of Force Generated by Individual Myosin Heads in Skinned Rabbit Psoas Muscle Fibers at Low Ionic Strength”, PLoS One. 2013 May 15: 8(5): e63658 |
2013年 | Takahiro Maruta, Takahiro Kobatake, Hiroyuki Okubo and Shigeru Chaen Single turnovers of fluorescence ATP bound to bipolar myosin filament during actinfilaments sliding. BIOPHYSICS 9: 13-20 (2013) |
2012年 | Haruo Sugi, Takakazu Kobayashi, Teizo Tsuchiya, Shigeru Chaen,Seiryo Sugiura Evidence for the essential role of myosin head lever arm domain and myosin subfragment-2 in muscle contraction. In Skeletal muscle-from myogenesis to clinical relations. (ed. J. Cseri) pp.125-140. INTECHOPEN.COM (2012) |
2012年 | Haruo Sugi, Takakazu Kobayashi, Teizo Tsuchiya, Shigeru Chaen,Seiryo Sugiura Evidence for the essential role of myosin head lever arm domain and myosin subfragment-2 in muscle contraction. In Skeletal muscle-from myogenesis to clinical relations. (ed. J. Cseri) pp.125-140. INTECHOPEN.COM (2012) |
2012年 | Haruo Sugi, Hiroki Minoda, Takuya Miyakawa, Suguru Tanokura, Shigeru Chaen, Takakazu Kobayashi The gas environmental chamber as a powerful tool to study structural changes of living muscle thick filaments coupled with ATP hydrolysis. In Current basic and pathological approaches to the function of muscle cells and tissues-From molecules to Human (ed. H. Sugi) pp.3-26. INTECHOPEN.COM (2012) |
2010年 | Hiroyuki Okubo, Masanori Iwai, Sosuke Iwai, Shigeru Chaen. Thermal activation energy for bidirectional movement of actin along bipolar tracks of myosin filaments.Biochemical and Biophysical Research Communications. 396(2):539-542. (2010) |
2010年 | Hideki Shishido, Kiyoshi Nakazato, Eisaku Katayama, Shigeru Chaen and Shinsaku Maruta Kinesin―Calmodulin fusion protein as a molecular shuttle. J.Biochem. 147(2):213-223. (2010) |
2008年 | Haruo Sugi, Hiroki Minoda, Y Inayoshi, F Yumoto, Takuya Miyakawa, Yumiko Miyauchi, Suguru Tanokura, Tsuyoshi Akimoto, Takakazu Kobayashi, Shigeru Chaen, Seiryo Sugiura Direct demonstration of the cross-bridge recovery stroke in muscle thick filaments in aqueous solution by using the hydration chamber. Proceedings of National Academy of Science 105, 17396-17401 (2008) |
2007年 | Iwai, S. and Chaen, S. Mutation in the SH1 helix reduces the activation energy of the ATP-induced conformational transition of myosin. Biochemical and Biophysical Research Communications 357, 325-329 (2007) |
2006年 | S., Hanamoto, D., and Chaen, S. A point mutation in the SH1 helix alters elasticity and thermal stability of myosin II.J. Biol. Chem. 281, 30736-30744. (2006) |
2004年 | yasi, T., Saeki, Y., Chaen, S., Shirakawa, I., and Sugi, H. :Effect of deuterium oxide on contraction charasteristics and ATPase activity in glycerinated single rabbit skeletal muscle fibers. Biochim. Biophys. Acta 1659, 46-51. (2004) |
2003年 | Chaen, S., Yamamoto, N., Shirakawa, I., and Sugi, H. :In vitro actomyosin motility in deuterium oxide. Adv. Exp. Med. Biol. 538, 183-192. (2003) |
2003年 | Sugi, H., and Chaen, S., :Force-velocity relationships in actin-myosin interactions causing cytoplasmic streaming in algal cells. J. Exp.Biol. 206, 1971-1976. (2003) |
2002年 | Maruta, S., Mizukura, Y., and Chaen, S., :Interaction of a new fluorescent ATP analogue with skeletal muscle myosin subfragment-1. J. Biochem. 131, 905-911. (2002) |
2001年 | Chaen, S., Yamamoto, N., Shirakawa, I., and Sugi, H. : Effect of deuterium oxide on actomyosin motility in vitro. Biochim. Biophys. Acta 1516, 218-223. (2001) |
2000年 | Shirakawa, I., Chaen, S., Bagshaw, C.R., and Sugi, H. :Measurement of nucleotide exchange rate constants in single rabbit soleus myofibrils during shortening and lengthening using a fluorescent ATP analog. Biophys. J. 78, 918-926. (2000) |
1998年 | Chaen, S., Shirakwa, I., Bagshaw, C.R., and Sugi, H. :Measurement of ATP turnover during shortening and lengthening of rabbit psoas myofibrils using a fluorescent ATP analog. Adv. Exp. Med. Biol. 453, 569-576. (1998) |
1998年 | Sugiura, S., Kobayakawa, N., Fujita, H., Momomura, S., Chaen, S., and Sugi, H. : Distinct kinetic properties of cardiac myosin isoforms revealed by in vitro studies. Adv. Exp. Med. Biol. 453, 125-130. (1998) |
1998年 | Watabe, S., Hirayama, Y., Nakaya, M., Kakinuma, M., Kikuchi, K., Guo, X-F., Katoh, S., Chaen, S., and Ooi, T. :Carp expresses fast skeletal myosin isoforms with altered motor functions and structural stabilities to compensate for changes in environmental temperature. J. Therm.Biol. 22, 375-390. (1998) |
1998年 | Sugi, H., Akimoto, T., Chaen, S., and Suzuki, S. :ATP-induced axial movement of myosin heads in living thick filaments recorded with a gas environmental chamber attached to the electron microscope. Adv. Exp. Med. Biol. 453, 53-61. (1998) |
1998年 | Miyata, H., Chaen, S., Sugiura, S., and Sugi, H. :Evanescent excitation microscopy. Its application to the study of single molecular process kinetics of actomyosin motor. Adv. Exp. Med. Biol. 453, 29-35. (1998) |
1998年 | Sugiura, S., Kobayakawa, N., Fujita, H., Yamashita, H., Momomura, S., Chaen, S., Omata, M., and Sugi, H. :Comparison of unitary displacements and forces between cardiac myosin isoforms by the optical trap technique:Molecular basis for cardiac adaptation. Circulation Res. 82, 1029-1034. (1998) |
1997年 | Chaen, S., Shirakawa, I., Bagshaw, C.R., and Sugi, H. :Measurement of nucleotide release kinetics in single skeletal myofibrils during isometric and isovelocity contractions using fluorescent microscopy. Biophys. J. 73, 2033-2042 (1997) |
1997年 | Sugi, H., Akimoto, T., Sutoh, K., Chaen, S., Oishi, N., and Suzuki, S. : Dynamic electron microscopy of ATP-induced myosin head movement in living muscle thick filaments.Proc. Natl. Acad. Sci. USA 94, 4378-4382. (1997) |
1996年 | Chaen, S., Nakaya, M., Guo, X-F., and Watabe, S. :Lower activation energy for sliding of F-actin on a less thermostable isoform of carp myosin. J. Biochem. 120, 788-791. (1996) |
1996年 | Sugiuara, S., Kobayakawa, N., Momomura, S., Chaen, S., Omata, M., and Sugi, H. : Different cardiac myosin isoforms exhibit equal force-generating ability in vitro. Biochim. Biophys. Acta 1273, 73-76. (1996) |
1995年 | Sugiuara, S., Yamashita, H., Sata, M., Momomura, S., Serizawa, T., Oiwa, K., Chaen, S., Shimmen, T., and Sugi, H. :Force-velocity relations of rat cardiac myosin isozymes sliding on algal cells actin cables in vitro. Biochim. Biophys. Acta 1231, 69-75. (1995) |
1995年 | Chaen, S., Inoue, J., and Sugi, H. :The force-velocity relationship of the ATP-dependent actin-myosin sliding causing cytoplasmic streaming in algal cells, studied using a centrifugal microscope. J. Exp. Biol. 198, 1021-1027 (1995) |
1995年 | Shirakwa, I., Oiwa, K., Chaen, S., Shimizu, T., Tanaka, H., and Sugi, H. :The mode of ATP-dependent microtuble-kinesin sliding in the auxotonic condition. J. Exp. Biol. 198, 1809-1815. (1995) |
1995年 | Kobayashi, T., Gross, T., Chaen, S., Sugiura, S., Tanaka, H., and Sugi, H. :Effect of lateral forces on the movement of myosin-coated beads on actin cables studied using a centrifugal microscope. Jpn. J. Physiol. 45, 177-186. (1995) |
1993年 | regear, R., Oiwa, K., Chaen, S., and Sugi, H. :Relation between magnetically-applied force and velocity in beads coated with rabbit myosin sliding on actin cables in Nitellopsis cells. J. Muscle Res. Cell Motil. 14, 412-415. (1993) |
1993年 | Sugi, H., Oiwa, K., and Chaen, S. : Dependent of the work done by ATP-induced actin-myosin sliding on the initial baseline force: its implications for kinetic properties of myosin heads in muscle contraction. Adv. Exp. Med. Biol. 332, 303-311. (1993) |
1993年 | Chaen, S., Oiwa, K., Kobayashi, T., Gross, T., Kamitubo, E., Shimmen, T. and Sugi, H. :Kinetic properties of the ATP-dependent actin-myosin sliding as revealed by the force-movement assay system with a centrifuge microscope. Adv. Exp. Med. Biol. 332, 351-360. (1993) |
1991年 | Oiwa, K., Chaen, S., and Sugi, H. :Measurement of work done by ATP-induced sliding between rabbit muscle myosin and algal cell actin cables in vitro. J. Physiol. 437, 751-763. (1991) |
1990年 | Oiwa, K., Chaen, S., Kamitsubo, E., Shimmen, T., and Sugi, H. :Steady-state force-velocity relation in the ATP-dependent sliding movement of myosin-coated beads on actin cables in vitro studied with a centrifuge microscope. Proc. Natl. Acad. Sci. USA 87, 7893-7897. (1990) |
1990年 | Kohno, T., Chaen, S., and Shimmen, T. :Characterization of the translocator associated pollen tube organelles. Plotoplasma 154, 179-183. (1990) |
1989年 | Chaen, S., Oiwa, K., Shimmen, T., Iwamoto, H., and Sugi, H. :Simultaneous recordings of force and sliding movement between myosin-coated glass microneedles and actin cables in vitro. Proc. Natl. Acad. Sci. USA 86, 1510-1514. (1989) |
1988年 | Chaen, S., Shimada, M., and Sugi, H. :Cooperative interactions of myosin heads in muscle force generation. Adv. Exp. Med. Biol. 226, 289-298. (1988) |
1986年 | Chaen, S., Shimada, M., and Sugi, H. :Evidence for cooperative interactions of myosin heads with thin filament in the force generation of vertebrate skeletal muscle fibers. J. Biol. Chem. 261, 13632-13636. (1986) |
1985年 | Chaen, S., Shimada, M., and Sugi, H. :Fluorescence properties and contraction characteristics of ANM(N-(1-anilinonaphtyl-4)maleimide)-labeled rabbit psoas muscle fiber. J. Biochem. 98, 939-947. (1985) |
1984年 | Gomi, S., Chaen, S., and Sugi, H. :The mode of action of maitotoxin on the membrane systems of frog skeletal muscle fibers. Proc. Japan Acad. 60, 28-31. (1984) |
1984年 | Chaen, S. and Shimizu, H. :Substrate-concentration dependence of contraction parameters in glycerinated insect flight msucle fibers from Lethocerus derollei. J. Biochem. 95, 839-845. (1984) |
1982年 | Chaen, S. and Sugi, H. :Substrate concentration dependence of contraction characteristics in glycerinated horseshoe crab muscle fibers. Comp. Biochem. Physiol. 72A, 623-626. (1982) |
1981年 | Chaen, S., Kometani, K., Yamada, T., and Shimizu, H. :Substrate-concentration dependence of tension, shortening velocity and ATPase activity of glucerinated single muscle fibers. J. Biochem. 90, 1611-1621. (1981) |
1977年 | Nishiyama, K., Shimizu, H., Kometani, K., and Chaen, S. :The three state model for the elementary process of energy conversion in muscle. Biochim. Biophys. Acta 460, 523-536 (1977) |
平成18〜19年度 | 基盤研究 (C) 基礎医学・生理学一般 課題番号18590210 遺伝性難聴DFNA17につながる変異ミオシンの運動機能解析 |
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平成14〜15年度 | 基盤研究 (C) 複合領域・生物物理学・分子生理学 課題番号14580678 ADP結合非筋ミオシンクロスブリッジの1分子計測 |
平成10〜11年度 | 基盤研究(C) 複合領域・生物物理学・分子生理学 課題番号10680641 アクトミオシンモーターFENN効果の一分子計測 |
平成10年度 | 特定領域研究(A)生物分子モーター 公募班班員 課題番号10175219 アクトミオシンモーター分子のストレイン依存ヌクレオチド結合状態の寿命解析 |
平成9年度 | 重点領域研究(2)生物分子モーター 公募班班員 課題番号09279226 アクトミオシンモーター分子のストレイン依存ADP結合状態の寿命解析 |
平成6年度 | 総合研究(A) 課題番号05305007 マイクロマシンとしての筋フィラメント滑り機構の動的特性に関する総合的研究 研究分担者 |
平成3年度 | 重点領域研究 課題番号03223101 滑り運動の多様性と統一性 計画班班員 |
平成2年度 | 重点領域研究 課題番号02239101 滑り運動の多様性と統一性 計画班班員 |
平成元年度 | 重点領域研究 課題番号01657001 滑り運動の多様性と統一性 計画班班員 |
昭和64年度 | 奨励研究(A) 医学・生理・生理学一般 課題番号01770065 ガラスマイクロニードルを用いたアクチンミオシンATP反応サイクル中の力発生検出 |
昭和63年度 | 奨励研究(A) 複合領域・生物物理学・分子生理学 課題番号63780324光ビームスプリット方式微小張力検出器による筋原線維の張力のゆらぎ解析 |
昭和62年度 | 奨励研究(A) 医学・生理・生理学一般 課題番号62770077 ミオシン線維を部分的に溶解した筋原線維の短縮のゆらぎ解析 |
昭和59年度 | 奨励研究(A) 複合領域・生物物理学・分子生理学 課題番号59780270 光アフィニティラベリング法によるミオシン分子双頭間の協同性の解析 |
昭和56年度 | 奨励研究(A) 複合領域・生物物理学・分子生理学 課題番号56790178 ANM蛍光試薬による筋線維内ATP分解と張力との関係の研究 |