日本大学 文理学部 物理生命システム科学科

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2014年 Haruo Sugi, Shigeru Chaen, Takakazu Kobayashi, Takahiro Abe, Kazushige Kimura, Yasutake Saeki, Yoshiki Ohnuki, Takuya Miyakawa, Masaru Tanokura, Seiryo SugiuraDefinite Differences between In Vitro Actin-Myosin Sliding and Muscle Contraction as Revealed Using Antibodies to Myosin Head. PLos One. 2014 9(6): e93272
2013年 Haruo Sugi, Takahiro Abe, Takakazu Kobayashi, Shigeru Chaen, Yoshiki Ohnuki, Yasutake Saeki, Seiryo SugiuraEnhancement of Force Generated by Individual Myosin Heads in Skinned Rabbit Psoas Muscle Fibers at Low Ionic Strength”, PLoS One. 2013 May 15: 8(5): e63658
2013年 Takahiro Maruta, Takahiro Kobatake, Hiroyuki Okubo and Shigeru Chaen
Single turnovers of fluorescence ATP bound to bipolar myosin filament during actinfilaments sliding. BIOPHYSICS 9: 13-20 (2013)
2012年 Haruo Sugi, Takakazu Kobayashi, Teizo Tsuchiya, Shigeru Chaen,Seiryo Sugiura Evidence for the essential role of myosin head lever arm domain and myosin
subfragment-2 in muscle contraction. In Skeletal muscle-from myogenesis to clinical relations. (ed. J. Cseri) pp.125-140. INTECHOPEN.COM (2012)
2012年 Haruo Sugi, Takakazu Kobayashi, Teizo Tsuchiya, Shigeru Chaen,Seiryo Sugiura Evidence for the essential role of myosin head lever arm domain and myosin
subfragment-2 in muscle contraction. In Skeletal muscle-from myogenesis to clinical relations. (ed. J. Cseri) pp.125-140. INTECHOPEN.COM (2012)
2012年 Haruo Sugi, Hiroki Minoda, Takuya Miyakawa, Suguru Tanokura, Shigeru Chaen, Takakazu Kobayashi The gas environmental chamber as a powerful tool to study structural changes of living muscle thick filaments coupled with ATP hydrolysis. In Current basic and pathological approaches to the function of muscle cells and tissues-From molecules to Human (ed. H. Sugi) pp.3-26. INTECHOPEN.COM (2012)
2010年 Hiroyuki Okubo, Masanori Iwai, Sosuke Iwai, Shigeru Chaen.
Thermal activation energy for bidirectional movement of actin along bipolar tracks of myosin filaments.Biochemical and Biophysical Research Communications. 396(2):539-542. (2010)
2010年 Hideki Shishido, Kiyoshi Nakazato, Eisaku Katayama, Shigeru Chaen and Shinsaku Maruta Kinesin―Calmodulin fusion protein as a molecular shuttle.
J.Biochem. 147(2):213-223. (2010)
2008年 Haruo Sugi, Hiroki Minoda, Y Inayoshi, F Yumoto, Takuya Miyakawa, Yumiko Miyauchi, Suguru Tanokura, Tsuyoshi Akimoto, Takakazu Kobayashi, Shigeru Chaen, Seiryo Sugiura
Direct demonstration of the cross-bridge recovery stroke in muscle thick filaments in aqueous solution by using the hydration chamber.
Proceedings of National Academy of Science 105, 17396-17401 (2008)
2007年 Iwai, S. and Chaen, S.
Mutation in the SH1 helix reduces the activation energy of the ATP-induced conformational transition of myosin.
Biochemical and Biophysical Research Communications 357, 325-329 (2007)
2006年 S., Hanamoto, D., and Chaen, S.
A point mutation in the SH1 helix alters elasticity and thermal stability of myosin II.J. Biol. Chem. 281, 30736-30744. (2006)
2004年 yasi, T., Saeki, Y., Chaen, S., Shirakawa, I., and Sugi, H.
:Effect of deuterium oxide on contraction charasteristics and ATPase activity in
glycerinated single rabbit skeletal muscle fibers.
Biochim. Biophys. Acta 1659, 46-51. (2004)
2003年 Chaen, S., Yamamoto, N., Shirakawa, I., and Sugi, H.
:In vitro actomyosin motility in deuterium oxide.
Adv. Exp. Med. Biol. 538, 183-192. (2003)
2003年 Sugi, H., and Chaen, S.,
:Force-velocity relationships in actin-myosin interactions causing cytoplasmic
streaming in algal cells.
J. Exp.Biol. 206, 1971-1976. (2003)
2002年 Maruta, S., Mizukura, Y., and Chaen, S.,
:Interaction of a new fluorescent ATP analogue with skeletal muscle myosin subfragment-1.
J. Biochem. 131, 905-911. (2002)
2001年 Chaen, S., Yamamoto, N., Shirakawa, I., and Sugi, H.
: Effect of deuterium oxide on actomyosin motility in vitro.
Biochim. Biophys. Acta 1516, 218-223. (2001)
2000年 Shirakawa, I., Chaen, S., Bagshaw, C.R., and Sugi, H.
:Measurement of nucleotide exchange rate constants in single rabbit soleus myofibrils
during shortening and lengthening using a fluorescent ATP analog.
Biophys. J. 78, 918-926. (2000)
1998年 Chaen, S., Shirakwa, I., Bagshaw, C.R., and Sugi, H.
:Measurement of ATP turnover during shortening and lengthening of rabbit psoas myofibrils using a fluorescent ATP analog.
Adv. Exp. Med. Biol. 453, 569-576. (1998)
1998年 Sugiura, S., Kobayakawa, N., Fujita, H., Momomura, S., Chaen, S., and Sugi, H.
: Distinct kinetic properties of cardiac myosin isoforms revealed by in vitro studies.
Adv. Exp. Med. Biol. 453, 125-130. (1998)
1998年 Watabe, S., Hirayama, Y., Nakaya, M., Kakinuma, M., Kikuchi, K., Guo, X-F., Katoh, S., Chaen, S., and Ooi, T.
:Carp expresses fast skeletal myosin isoforms with altered motor functions and structural stabilities to compensate for changes in environmental temperature. J. Therm.Biol. 22, 375-390. (1998)
1998年 Sugi, H., Akimoto, T., Chaen, S., and Suzuki, S.
:ATP-induced axial movement of myosin heads in living thick filaments recorded with a gas environmental chamber attached to the electron microscope.
Adv. Exp. Med. Biol. 453, 53-61. (1998)
 1998年 Miyata, H., Chaen, S., Sugiura, S., and Sugi, H.
:Evanescent excitation microscopy. Its application to the study of single molecular process kinetics of actomyosin motor.
Adv. Exp. Med. Biol. 453, 29-35. (1998)
1998年 Sugiura, S., Kobayakawa, N., Fujita, H., Yamashita, H., Momomura, S., Chaen, S., Omata, M., and Sugi, H.
:Comparison of unitary displacements and forces between cardiac myosin isoforms by the optical trap technique:Molecular basis for cardiac adaptation.
Circulation Res. 82, 1029-1034. (1998)
1997年 Chaen, S., Shirakawa, I., Bagshaw, C.R., and Sugi, H.
:Measurement of nucleotide release kinetics in single skeletal myofibrils during isometric and isovelocity contractions using fluorescent microscopy.
Biophys. J. 73, 2033-2042 (1997)
1997年 Sugi, H., Akimoto, T., Sutoh, K., Chaen, S., Oishi, N., and Suzuki, S.
: Dynamic electron microscopy of ATP-induced myosin head movement in living muscle thick filaments.Proc. Natl. Acad. Sci. USA 94, 4378-4382. (1997)
1996年 Chaen, S., Nakaya, M., Guo, X-F., and Watabe, S.
:Lower activation energy for sliding of F-actin on a less thermostable isoform of carp myosin.
J. Biochem. 120, 788-791. (1996)
 1996年 Sugiuara, S., Kobayakawa, N., Momomura, S., Chaen, S., Omata, M., and Sugi, H.
: Different cardiac myosin isoforms exhibit equal force-generating ability in vitro.
Biochim. Biophys. Acta 1273, 73-76. (1996)
 1995年 Sugiuara, S., Yamashita, H., Sata, M., Momomura, S., Serizawa, T., Oiwa, K., Chaen, S., Shimmen, T., and Sugi, H.
:Force-velocity relations of rat cardiac myosin isozymes sliding on algal cells actin cables in vitro.
Biochim. Biophys. Acta 1231, 69-75. (1995)
 1995年 Chaen, S., Inoue, J., and Sugi, H.
:The force-velocity relationship of the ATP-dependent actin-myosin sliding causing cytoplasmic streaming in algal cells, studied using a centrifugal microscope.
J. Exp. Biol. 198, 1021-1027 (1995)
 1995年 Shirakwa, I., Oiwa, K., Chaen, S., Shimizu, T., Tanaka, H., and Sugi, H.
:The mode of ATP-dependent microtuble-kinesin sliding in the auxotonic condition.
J. Exp. Biol. 198, 1809-1815. (1995)
 1995年 Kobayashi, T., Gross, T., Chaen, S., Sugiura, S., Tanaka, H., and Sugi, H.
:Effect of lateral forces on the movement of myosin-coated beads on actin cables studied using a centrifugal microscope.
Jpn. J. Physiol. 45, 177-186. (1995)
1993年 regear, R., Oiwa, K., Chaen, S., and Sugi, H.
:Relation between magnetically-applied force and velocity in beads coated with rabbit myosin sliding on actin cables in Nitellopsis cells.
J. Muscle Res. Cell Motil. 14, 412-415. (1993)
1993年 Sugi, H., Oiwa, K., and Chaen, S.
: Dependent of the work done by ATP-induced actin-myosin sliding on the initial baseline force: its implications for kinetic properties of myosin heads in muscle contraction.
Adv. Exp. Med. Biol. 332, 303-311. (1993)
1993年 Chaen, S., Oiwa, K., Kobayashi, T., Gross, T., Kamitubo, E., Shimmen, T. and Sugi, H.
:Kinetic properties of the ATP-dependent actin-myosin sliding as revealed by the force-movement assay system with a centrifuge microscope.
Adv. Exp. Med. Biol. 332, 351-360. (1993)
1991年 Oiwa, K., Chaen, S., and Sugi, H.
:Measurement of work done by ATP-induced sliding between rabbit muscle myosin and algal cell actin cables in vitro.
J. Physiol. 437, 751-763. (1991)
1990年 Oiwa, K., Chaen, S., Kamitsubo, E., Shimmen, T., and Sugi, H.
:Steady-state force-velocity relation in the ATP-dependent sliding movement of myosin-coated beads on actin cables in vitro studied with a centrifuge microscope.
Proc. Natl. Acad. Sci. USA 87, 7893-7897. (1990)
1990年 Kohno, T., Chaen, S., and Shimmen, T.
:Characterization of the translocator associated pollen tube organelles.
Plotoplasma 154, 179-183. (1990)
1989年 Chaen, S., Oiwa, K., Shimmen, T., Iwamoto, H., and Sugi, H.
:Simultaneous recordings of force and sliding movement between myosin-coated glass microneedles and actin cables in vitro.
Proc. Natl. Acad. Sci. USA 86, 1510-1514. (1989)
1988年 Chaen, S., Shimada, M., and Sugi, H.
:Cooperative interactions of myosin heads in muscle force generation.
Adv. Exp. Med. Biol. 226, 289-298. (1988)
1986年 Chaen, S., Shimada, M., and Sugi, H.
:Evidence for cooperative interactions of myosin heads with thin filament in the force generation of vertebrate skeletal muscle fibers.
J. Biol. Chem. 261, 13632-13636. (1986)
1985年 Chaen, S., Shimada, M., and Sugi, H.
:Fluorescence properties and contraction characteristics of ANM(N-(1-anilinonaphtyl-4)maleimide)-labeled rabbit psoas muscle fiber.
J. Biochem. 98, 939-947. (1985)
1984年 Gomi, S., Chaen, S., and Sugi, H.
:The mode of action of maitotoxin on the membrane systems of frog skeletal muscle fibers.
Proc. Japan Acad. 60, 28-31. (1984)
1984年 Chaen, S. and Shimizu, H.
:Substrate-concentration dependence of contraction parameters in glycerinated insect flight msucle fibers from Lethocerus derollei.
J. Biochem. 95, 839-845. (1984)
1982年 Chaen, S. and Sugi, H.
:Substrate concentration dependence of contraction characteristics in glycerinated horseshoe crab muscle fibers.
Comp. Biochem. Physiol. 72A, 623-626. (1982)
1981年 Chaen, S., Kometani, K., Yamada, T., and Shimizu, H.
:Substrate-concentration dependence of tension, shortening velocity and ATPase activity of glucerinated single muscle fibers.
J. Biochem. 90, 1611-1621. (1981)
1977年 Nishiyama, K., Shimizu, H., Kometani, K., and Chaen, S.
:The three state model for the elementary process of energy conversion in muscle.
Biochim. Biophys. Acta 460, 523-536 (1977)

 

 

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